Journal: Journal of visualized experiments : JoVE

Article Title: Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

doi: 10.3791/61491

Figure Lengend Snippet: Table of Materials

Article Snippet: Low Noise Cable for Microelectrode Holders , WPI Inc , 5372 , Suggested for improving the length and placement of the cables and electrode holder assemblies.

Techniques: Convection, Software, Eye Drops

Journal: Journal of visualized experiments : JoVE

Article Title: Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

doi: 10.3791/61491

Figure Lengend Snippet: (A) Image of the completed circuit accomplished by lowering the recording (glass capillary microelectrodes), reference, and ground electrodes into the same bath solution. This configuration enables preliminary tests to be run (prior to anesthetizing the mouse) to evaluate the characteristic impedance, noise, and drift. Inset (upper left) showing a side-view schematic of the custom microelectrode holder stand. (B) Representative image of the Impedance Checking Mode showing the appropriate values for electrode impedances. The impedance in the Left and Right eye electrodes should be comparable, within 5 KΩ of each other (e.g., Left eye: 38.7 KΩ vs. Right eye: 40.36 KΩ). The impedance of the mouth reference electrode should be less than 1 KΩ, whereas the tail electrode should be around 2.5 KΩ. (C) Representative image of the preview trace (Step 4/6) is shown. Step 4 (Long Flash No Light) is selected as no light is delivered during the preview of this step. The traces should be low noise and may have a slight drift that gradually fades with time to baseline. Once the traces have achieved a constant drift in both channels and become relatively flat, the actual recording can begin. (D) Using Step 5/6 (Long Flash 10 cd 7 min) after 0.5 min of darkness, a light step of 10 cd/m2 is delivered to the mouse for 7 min followed by a return to darkness for 1.5 min. (E) Image of the export parameters used to convert the data to a *.csv file. This precise format is required to run the DC-ERG analysis software.

Article Snippet: Low Noise Cable for Microelectrode Holders , WPI Inc , 5372 , Suggested for improving the length and placement of the cables and electrode holder assemblies.

Techniques: Software